The Barad lab seeks a postdoctoral scholar to investigate cytoskeletal reorganization during intracellular bacterial infection using cellular cryo-electron tomography. The scholar will receive training in sample preparation, tilt series collection, and data analysis for cryo-electron tomography, as well as development of computational tools for the quantitative analysis of membrane and filament organization. In addition to cryo-electron tomography, the project will incorporate correlative cryo-fluorescence microscopy as well as live-cell fluorescence microscopy.
Applicants should send a cover letter describing research experience and interests, a CV, and contacts for references to Benjamin Barad (barad@ohsu.edu).
The Barad lab explores mechanisms of mammalian membrane remodeling and organellar reorganization during bacterial infection. The primary technique used in the lab is cellular cryo-electron tomography, and the lab is invested in methods development advancing tomography as a scale-spanning technique for contextual structural and cell biology. Projects in the lab may also involve classical structural biology experiments (primarily single-particle cryo-EM and x-ray crystallography), light microscopy, volume EM, and biophysical characterization. Most projects in the lab involve significant computational analysis. Preference will be given to applicants with expertise in either cryo-electron microscopy or tomography or computational image analysis.
Individuals are expected to work on-site to conduct sample preparation and light and electron microscopy experiments. Additional safety training will be needed for work with cell lines and bacterial infection experiments in a BSL-2 environment, and for cryogenic sample preparation and handling.
Personnel will need to work at lab benches, desks, computers and/or microscopes for up to 4 hours at a time. Ability to perform repetitive lab manipulations (gripping, grasping, reaching, pulling), move lab equipment under 25 lbs with accommodation, perform image analysis operation and help others with equipment use. Personal protective equipment must be worn as required. (Gloves, mask/respirator, eyewear, lab coat, etc).
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